Table 2.
The effect of inhibitors used on phenylephrine concentration-response curves in endothelium intact rings
| Vehicle | AG1478 | Inhibitor | AG1478+ inhibitor | |
|---|---|---|---|---|
| Heparin | ||||
| Emax (%) | 91.2 ± 2.8 | 76.8 ± 5.2a | 93.0 ± 2.6 | 64.2 ± 5.7a |
| pEC50 | 7.61 ± 0.14 | 6.64 ± 0.09a | 7.62 ± 0.12 | 6.40 ± 0.03a |
| GM6001 | ||||
| Emax (%) | 94.1 ± 1.7 | 85.1 ± 1.7a | 96.8 ± 1.8 | 79.3 ± 3.8a |
| pEC50 | 7.79 ± 0.15 | 6.92 ± 0.06a | 7.93 ± 0.12 | 7.02 ± 0.10a |
| ML5 | ||||
| Emax (%) | 91.7 ± 6.1 | 79.3 ± 3.8a | 92.9 ± 1.7 | 72.5 ± 1.6a |
| pEC50 | 7.41 ± 0.06 | 6.64 ± 0.09a | 7.48 ± 0.06 | 6.80 ± 0.12a |
| PD98059 | ||||
| Emax (%) | 88.4 ± 1.2 | 54.7 ± 10.6a | 69.8 ± 7.8 | 52.0 ± 9.2a |
| pEC50 | 7.06 ± 0.09 | 6.28 ± 0.06a | 6.80 ± 0.06 | 6.28 ± 0.10a |
| PP1 | ||||
| Emax (%) | 85.6 ± 2.9 | 55.0 ± 6.8a | 78.4 ± 5.3 | 60.5 ± 8.1a |
| pEC50 | 7.17 ± 0.10 | 6.36 ± 0.14a | 6.85 ± 0.11 | 6.30 ± 0.10a |
| Wortmannin | ||||
| Emax (%) | 85.6 ± 3.1 | 59.6 ± 8.3a | 58.3 ± 7.0a | 42.7 ± 6.6a |
| pEC50 | 7.26 ± 0.20 | 6.38 ± 0.15a | 6.83 ± 0.13 | 6.41 ± 0.17a |
| LY294002 | ||||
| Emax (%) | 82.7 ± 2.5 | 63.2 ± 7.3a | 66.8 ± 0.9a | 66.9 ± 3.1a |
| pEC50 | 7.25 ± 0.10 | 6.85 ± 0.07a | 7.00 ± 0.10 | 6.63 ± 0.08a |
Data are given as mean ± SEM.
a
P < 0.01 versus vehicle.
Emax, maximal contraction response in % of KCl; pEC50, negative logarithm of the EC50.