Figure 1.
Purification and preliminary pharmacological characterization of ρ-Da1b. (A) Ion-exchange chromatography of Dendroaspis angusticeps crude venom. Labelled peaks were collected (13 fractions). (B) Reverse-phase chromatography of fraction H on a Vydac C18 preparative column. Labelled peaks were collected (20 fractions). Fraction D was eluted at around 27% acetonitrile. (C) Reverse-phase chromatography of fraction D on a Vydac C18 analytical column. Arrows show active peaks. (D) Inhibition of 3H-rauwolscine (1 nM) binding on rat brain synaptosomes by the minor peak purified in C.