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Characterization of the mode of action of ρ-Da1b on α2A-adrenoceptor expressed on CHO cells. (A) Saturation binding experimentof 3H-rauwolscine in the absence (•) or presence of ρ-Da1b (○, 10 µM). (B) Dissociation kinetic rate of 3H-rauwolscine in the presence of rauwolscine (black), rauwolscine plus ρ-Da1b (red), ρ-Da1b (green) and rauwolscine + 5-(N-ethyl-N-isopropyl)-amiloride (EPA) (purple).
