Table 2.
Alignment of sequences for ρ-Da1b and various other toxins acting on adrenoceptors and muscarinic receptors
| Edman's degradation | LTXVTKDTIFGITTQNXPAGQNLXFIRRHYINHRYTEITRGXTATXPKPTNVRE------------ | ||
|---|---|---|---|
| Mass sequencing | -----------------–-----------------------------------ETIHCCNTDKCNE | ||
| Loops | -------1----- ---------2------- -----3----- | % | |
| ρ-Da1b | Da | LTCVTKDTIFGITTQNCPAGQNLCFIRRHYINHRYTEITRGCTATCPKPTNVRETIHCCNTDKCNE | |
| MT-α | Dpp | LTCVTSKSIFGITTENCPDGQNLCFKKWYYLNHRYSDITWGCAATCPKPTNVRETIHCCETDKCNE | 77 |
| MT3 | Da | LTCVTKNTIFGITTENCPAGQNLCFKRWHYVIPRYTEITRGCAATCPIPENY-DSIHCCKTDKCNE | 76 |
| MT4 | Da | LTCVTSKSIFGITTENCPDGQNLCFKKWYYIVPRYSDITWGCAATCPKPTNVRETIHCCETDKCNE | 76 |
| MT1 | Da | LTCVTSKSIFGITTENCPDGQNLCFKKWYYIVPRYSDITWGCAATCPKPTNVRETIRCCETDKCNE | 74 |
| MT-β | Dpp | LTCVTSKSIFGITTEDCPDGQNLCFKRRHYVVPKIYDITRGCVATCPIPENY-DSIHCCKTDKCNE | 67 |
| ρ-Da1a | Da | LTCVTSKSIFGITTEDCPDGQNLCFKRRHYVVPKIYDSTRGCAATCPIPENY-DSIHCCKTDKCNE | 66 |
| Cm3 | Dpp | LTCVTSKSIFGITTEDCPDGQNLCFKRRHYVVPKIYDITRGCVATCPIPENY-DSIHCCKTEKCNN | 64 |
| MT2 | Da | LTCVTTKSIGGVTTEDCPAGQNVCFKRWHYVTPKNYDIIKGCAATCPKVDNN-DPIRCCGTDKCND | 55 |
| MT7 | Da | LTCVKSNSIWFPTSEDCPPGQNLCFKRWQYISPRMYDFTRGCAATCPKAEYR-DVINCCGTDKCNK | 54 |
| β-ca | Oh | RKCLNTPLPLIYTT--CPIGQDKCVKMTIKKLPSKYDVIRGCIDICPKSSA-DVEVLCCDTNKCNK | 36 |
Percentage identity, relative to ρ-Da1b, is indicated for each sequence. Residues that differ from ρ-Da1b are in bold. Da, Dendroaspis angusticeps; Dpp, Dendroaspis polylepis polylepis; Nak, Naja kaouthia; β-Ca, β-cardiotoxin; Oh, Ophiophagus hannah.