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. 2010 Oct 11;20(1):40–50. doi: 10.1093/hmg/ddq430

Figure 7.

Figure 7.

The PINK1/parkin pathway rescues mitochondrial defects in DJ-1-deficient cells. (A) Mobile fraction from FRAP experiments shows rescue of loss of mitochondrial connectivity by parkin or PINK1 in DJ-1-deficient cells (red bars) or control shRNA cells (open bars). Statistical significance was calculated using one-way ANOVA with Newman–Keuls post hoc test; *P < 0.05, n = 30 cells per condition, representative of duplicate experiments. (B) Similar experiment in DJ-1 wild-type (open bars) or knockout (red bars) MEFs. Statistical significance was calculated using one-way ANOVA with Newman–Keuls post hoc test; ***P < 0.001, n = 30 cells per condition, representative of duplicate experiment. (C) Control (open bars) or PINK1-deficient (purple bars) M17 cells were transfected with DJ-1 and treated with 100 nm rotenone for 24 h as indicated. Statistical significance was calculated using one-way ANOVA with Newman–Keuls post hoc test; ***P < 0.001; **P < 0.01, n = 30 cells per condition, representative of duplicate experiments.