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. 2010 Oct 6;20(1):90–103. doi: 10.1093/hmg/ddq435

Figure 2.

Figure 2.

Co-purification of MLC1 and Na,K-ATPase by ouabain affinity chromatography. Fractions eluted from ouabain affinity chromatography of cytosol (A) and membrane (B) extracts from the rat brain. Chromatography fractions were analysed by SDS–PAGE and WB and probed with Abs against MLC1, Na,K-ATPase β1 (NaK-β1), early endosome antigen1 (EEA1), Kir4.1, syntrophin (synt), dystrobrevin (DB) and aquaporin 4 (AQP4). MLC1, NaK-β1, Kir4.1, synt and α1 and α2 dystrobrevin (DB) isoforms (87 and 55 kDa revealed by the monoclonal antibody), but not AQP-4 and EEA1, were detected in the ouabain eluate from the cytosolic extract of rat brain (A). No MLC1 was found in the membrane eluate, although NaK-β1, Kir4.1 and syntrophin were present (B).