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Recycling of the transferrin receptor in CT60 cells is improved by restoration of acid SMase activity. The efflux kinetics of [125I]-transferrin was measured in 25RA, CT60, CT60-VEC, CT60-WT and CT60-C629S cells expressing the hTfR, as described in Materials and Methods. The values for CT60-WT and CT60-C629S cells were significantly different from that of CT60 and CT60-VEC cells (p < 0.05).
