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. Author manuscript; available in PMC: 2011 Nov 18.
Published in final edited form as: Neuron. 2010 Nov 18;68(4):739–749. doi: 10.1016/j.neuron.2010.09.029

Figure 3. Reconstitution finds three P2X receptors that can mediate ASIC sensitization.

Figure 3

(A) Each trio of traces is from a single COS or CHO cell transfected with the indicated cDNAs. The ASIC3 currents, evoked by 2 second steps to pH 6.9 before ATP application and after removal, flank a trace showing the first 3 seconds of a 30 second ATP application. αβ-methylene-ATP was used on the P2X2/P2X3 co-transfection to assure only activation of the heteromer and not any P2X2 homomers. Scale bars: 1 sec; 0.5 nA. See supplemental Figure S3 for time course of sensitization in CHO cells. (B) Mean percent ASIC current increase (n = 7–83 cells, each from dishes not previously exposed to ATP) in sensory neurons (DRG) or cell lines transfected with ASIC3 or ASIC1 together with the indicated P2X receptor(s). P2X2, P2X4, and P2X5 can mediate sensitization of ASIC3; ASIC1 also responds to ATP.