Figure 5.

3HV biosynthesis solely from glucose. This figure shows shake-flask production of chiral 3HV in various knock-out strains as described in Table 1. Cells were grown in LB supplemented with 20 g/L glucose. The top and bottom dashed lines represent the acetate titers produced from E. coli strain HCT 10 and HCT 20 harboring empty plasmids, respectively. All strains contained the same set of plasmids pET-PB-B, pCOLA-Tecm-Icg, and pCDF-T-H (for (S)-3HV synthesis) or pCDF-T-P (for (R)-3HV synthesis). The recombinant HCT 10 strains carrying an empty pCOLAduet-1 in place of pCOLA-Tecm-Icg, as control strains, produced essentially no 3HV (data not shown).