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Determination of the stereochemistry of 3HV. HPLC spectra of (A) racemic 3HV standards after boiling in methanol, (B) culture medium from the recombinant strain HCT 10 expressing bktB, phaB, tesB, and ptb-buk after boiling in methanol, and (C) culture medium from the recombinant strain HCT 10 expressing bktB, hbd, tesB, and ptb-buk after boiling in methanol are shown.
