Table 3.

Relative differences in cytokine amounts between L. plantarum WCFS1 wild-type and deletion mutants.

		IL-10c			IL-12			IL-10/IL-12
Mutant comparisona	Growth phaseb	value	p-value	adj. p- value	value	p-value	adj. p- value	value	p-value	adj. p- value
lp_1953	log	0.097	0.461	0.830	-0.041	0.775	0.825	0.138	0.161	0.803
	stat	0.253	0.057	0.228	-0.043	0.761	0.825	0.296	0.003	0.024 *
pts19ADCBR	log	0.164	0.216	0.647	0.106	0.458	0.825	0.058	0.556	0.923
	stat	0.396	0.004	0.031 *	-0.131	0.371	0.825	0.529	0.000	0.000 ***
plnEFI	log	0.287	0.031	0.176	0.032	0.825	0.825	0.255	0.010	0.071
	stat	0.344	0.010	0.071	0.174	0.225	0.825	0.170	0.084	0.507
plnG	log	0.280	0.035	0.176	-0.070	0.625	0.825	0.350	0.000	0.005 **
	stat	-0.028	0.830	0.830	-0.146	0.307	0.825	0.118	0.230	0.921
lamA lamR	log	0.511	0.000	0.001 ***	0.199	0.165	0.825	0.312	0.002	0.016 *
	stat	1.331	0.000	0.000 ***	1.321	0.000	0.000 ***	0.009	0.923	0.923

^a L. plantarum WCFS1 deletion mutant measured in the PBMC assay.

^b Phase of growth from which L. plantarum cells were harvested (log = exponential phase; stat = stationary phase).

^c The value is the average difference in ²Log cytokine amounts induced by wild-type L. plantarum and mutant cells harvested in the same phase of growth (log or stat). A positive value indicates an increase in IL-10 levels produced by PBMCs in response to mutant L. plantarum compared to the wild-type cells. Calculations of t-test p-values and adjusted (adj.) p-values are described in the text (Materials and Methods). * (0.01 < p < 0.05); ** (0.002 < p < 0.01); *** (p < 0.002) for the adj. p-values.