Table 4.
Primers used in this study.
| Primer | Sequencea |
|---|---|
| LF1953F | 5'- TGCCGCATACCGAGTGAGTAG-3' |
| LF1953R | 5'-CGAACGGTAGATTTAAATTGTTTATCAAAAAACACCGTTAATTTGCATC-3' |
| RF1953F | 5'-GTACAGCCCGGGCATGAGCGTGGCCATTAGTTGACGAGAC-3' |
| RF1953R | 5'-AACGCCATCGCACTGATGCATC-3' |
| Ecl-loxR | 5'-AAACAATTTAAATCTACCGTTCG-3' |
| Pml-loxF | 5'-CTCATGCCCGGGCTGTAC-3' |
| LF1953F2 | 5'-GCAACGGCTGTCAGTAACCTGCCTTC-3' |
| RF1953R2 | 5'-TCAAATCTCGAAGCGGTTCAAAACTG-3' |
| LF2647F | 5'-GTACAGCCCGGGCATGAGGGTATTTAGCGAAATATACAGATTG-3' |
| LF2647R | 5'-CTTTAGCCGTCTCATTAGTCG-3' |
| RF2651F | 5'-GGATTACCAAAACGAACATGG-3' |
| RF2651R | 5'-CGAACGGTAGATTTAAATTGTTTACTAGCCATTTTGTTTTTATCTCC-3' |
| LF2647R2 | 5'-TGACATGACTATCCTGACTTGC-3' |
| RF2651F2 | 5'-AACGTTCAACGGCAGATAAGCC-3' |
| LF423F | 5'-AATTGATACATGTGGTTTCGAAAG-3' |
| LF423R | 5'-CGAACGGTAGATTTAAATTGTTTCCAATGCATACTTGTACTCCC-3' |
| RF423F | 5'-GTACAGCCCGGGCATGAG CGACTTGATCAATAGCTGAGGG-3' |
| RF423R | 5'-TTGGTTGCCTTGATCGTGTAAG-3' |
| LF423F2 | 5'-CTTCAGTTATCGCTACAATCAACG-3' |
| RF423R2 | 5'-ACTAACGTACTTTGCACCACGG-3' |
| LF419F | 5'-GTACAGCCCGGGCATGAGGACGAGTAATCATCCATTCTGA-3' |
| LF419R | 5'-ATGAGTTTGCAATGGAGCTTAGG-3' |
| RF422F | 5'-CAAAGACGTGCCGAATATAGCC-3' |
| RF422R | 5'-CGAACGGTAGATTTAAATTGTTTAAACTGTAGCATAAATAATCCCC-3' |
| LF419R2 | 5'-GAGATAATTATTGTAAGACCGTC-3' |
| RF422F2 | 5'-CTAACGCATCAATAATCTTACTGG-3' |
a Bold and underlined nucleotides signify overlapping ends with the Ecl-loxR and Pml-loxF primers.