Table 1.
Comparison of Gliding Rates and Sporulation for mgl mutants
| Gliding on | Sporulation | |||
|---|---|---|---|---|
| A-motilitya | S-motilityb | Percent of WTc | ||
| Strain | Genotype | Average Speed in μm/min (Minutes per reversal) | ||
| WT | DK1622 | 2.6 (20.7) | 4.8 (15.6) | 100 ± 20 |
| ΔmglBA | DK6204 | NM | 1.9 (10.3) | < 0.01 |
| ΔmglBA+mglBA+ | MxH2419 | 2.1 (14.8) | 5.3 (10.8) | 100 ± 6 |
| ΔmglBA+mglBAG19A | MxH2445 | NM | 2.7 (11.8) | < 0.01 |
| ΔmglBA+mglBAG21V | MxH2361 | NM | 2.8 (11.8) | 0.01 ± 0.01 |
| ΔmglBA+mglBAL22V | MxH2359 | 1.9 (20.6) | 3.8 (12.0) | 15 ± 4 |
| ΔmglBA+mglBAK25A | MxH2430 | NM | 2.7 (10.5) | < 0.01 |
| ΔmglBA+mglBAT26N | MxH2410 | NM | 1.4 (11.3) | < 0.01 |
| ΔmglBA+mglBAD52A | MxH2408 | NM | 1.1 (10.3) | < 0.01 |
| ΔmglBA+mglBAT54A | MxH2406 | NM | 2.0 (10.3) | < 0.01 |
| ΔmglBA+mglBAT78A | MxH2247 | 0.7 (15.5) | 3.0 (11.5) | 15 ± 3 |
| ΔmglBA+mglBAT78S | MxH2248 | 1.4 (21.8) | 2.7 (7.8) | < 0.01 |
| ΔmglBA+mglBAT78D | MxH2432 | NM | NM | 0.1 ± 0.0 |
| ΔmglBA+mglBAP80A | MxH2357 | NM | NM | 20 ± 6 |
| ΔmglBA+mglBAQ82A | MxH2320 | NM | 2.0 (8.0) | < 0.01 |
| ΔmglBA+mglBAQ82R | MxH2319 | NM | 1.8 (10.3) | 0.01 ± 0.0 |
| ΔmglBA+mglBAL117/L120A | MxH2339 | NM | 1.4 (9.7) | < 0.01 |
| ΔmglBA+mglBAL124K | MxH2279 | 3.6 (8.4) | 5.0 (7.6) | < 0.01 |
| ΔmglBA+mglBAN141A | MxH2338 | NM | 1.8 (9.8) | < 0.01 |
| ΔmglBA+mglBAK142A | MxH2365 | NM | 2.5 (10.2) | < 0.01 |
| ΔmglBA+mglBAD144A | MxH2367 | NM | 1.6 (10.6) | < 0.01 |
| WT + mglBA+ | MxH2375 | 2.1 (9.7) | 8.9 (16.0) | 40 ± 10.0 |
| WT + mglB+ | MxH2391 | 2.3 (20.0) | 6.6 (15.0) | 40 ± 10.0 |
| WT+mglBAG19A | MxH2431 | 1.3 (20.8) | 4.0 (19.7) | 10 ± 0.6 |
| WT+mglBAG21V | MxH2360 | 2.1 (18.2) | 5.2 (15.3) | 100 ± 12 |
| WT+mglBAL22V | MxH2358 | 1.8 (15.3) | 7.6 (17.5) | 2 ± 1.5 |
| WT+mglBAK25A | MxH2429 | 1.8 (21.3) | 5.2 (13.6) | 60 ± 15 |
| WT+mglBAT26N | MxH2409 | 1.9 (21.0) | 8.3 (12.5) | < 0.01 |
| WT+mglBAD52A | MxH2407 | NM | 8.7 (12.4) | 0.03 ± 0.01 |
| WT+mglBAT54A | MxH2405 | 2.5 (16.2) | 9.3 (14.4) | 0.01 ± 0.0 |
| WT+mglBAT78A | MxH2425 | 1.7 (25.0) | 8.2 (13.4) | 30 ± 6 |
| WT+mglBAT78S | MxH2426 | 2.2 (21.4) | 7.1 (15.5) | < 0.01 |
| WT+mglBAT78D | MxH2428 | NM | 6.0 (12.6) | 90 ± 5 |
| WT+mglBAP80A | MxH2356 | 2.0 (23.6) | 2.3 (18.3) | 40 ± 6 |
| WT+mglBAQ82A | MxH2404 | 1.6 (30.0) | 7.5 (13.5) | < 0.01 |
| WT+mglBAQ82R | MxH2368 | 2.6 (22.1) | 10.0 (22.2) | 100 ± 18 |
| WT+mglBAL117/L120A | MxH2337 | 1.3 (15.6) | 8.1 (18.4) | 100 ± 18 |
| WT+mglBAL124K | MxH2278 | 2.4 (15.1) | 3.5 (15.4) | < 0.01 |
| WT+mglBAN141A | MxH2336 | 1.7 (NR) | 2.1 (17.2) | 0.2 ± 0.2 |
| WT+mglBAK142A | MxH2364 | 1.4 (21.3) | 9.3 (17.6) | 40 ± 6 |
| WT+mglBAD144A | MxH2366 | 1.6 (22.5) | 2.4 (11.5) | 4 ± 1 |
Time-lapse microscopy was performed to determine the rates of gliding cells.
a Gliding and reversal rates for cells using A-motility were measured on 1.5% CTPM agarose pads as described in Methods. NM = Cells were nonmotile. NR = no reversals observed.
b Gliding and reversal rates for cells using S-motility were measured in 0.5% methylcellulose plus 0.5× CTPM as described in Methods. NM = Cells were nonmotile. Gliding speeds are represented as the average and range of 25 cells from two independent assays.
cSporulation rates are given as a percentage relative to the WT and the standard deviation if available. The ability of MglA mutants to complement the sporulation defects of the ΔmglBA mutant was performed as described in Methods. mgl alleles were introduced into the WT background to determine MglA mutants could interfere with the function of normal MglA during sporulation.