Fig. 5.
Circulating DNA levels are lower in TLR9 mutant mice following HS/T. WT and TLR9 mutant mice were subjected to bilateral femur fracture and hemorrhagic shock (HS/T), or sham procedure. Plasma double-stranded (ds) DNA (A) and single-stranded (ss) DNA (B) levels were measured for analysis of DNA release following injury. TLR9 mutant mice subjected to HS/T showed significantly lower cell-free plasma dsDNA and ssDNA levels as compared with WT (*, P<0.05; **, P<0.01). Data represent means ± SEM; n = 6–8 mice group.