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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Oncogene. 2010 Sep 6;30(1):117–126. doi: 10.1038/onc.2010.403

Figure 3. TPEN a zinc specific chelator blocks apoptosis by MI-219.

Figure 3

[A] Cells were treated with MI-219 at indicated concentration for 72 hrs. TPEN blocked MI 219-induced apoptosis (Histone/DNA ELISA) which could be restored by addition of physiological levels (16 μM) of ZnCl2. [B] Zinc augments MI-219-induced apoptosis (Annexin V FITC assay) which can be abrogated by TPEN. Depletion of calcium using the calcium specific chelator, Bapta-AM or copper, using a specific copper chelator, bathocuproine, had no effect on MI-219-induced apoptosis in either cell line. All experiments were performed in triplicate, data presented represents mean of three independent experiments. * represents p<0.05 and ** p<0.01.