Figure 3.

Relative affinities for trypsin of a series of MCoTI-I mutants covering all the loop positions except loop 6 and Cys residues. A model of cyclotide MCoTI-I bound to trypsin is shown at the bottom indicating the position of the mutations. The side-chain of residue K6 is shown in red bound to specificity pocket of trypsin. The model was produced by homology modeling at the Swiss model workspace⁴¹ using the structure of CPTI-II-trypsin complex (PDB code: 2btc)⁴² as template. Structure was generated using the PyMol software package. Figure adapted from reference.¹⁶