Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 29;285(51):39580–39587. doi: 10.1074/jbc.M110.171850

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 1. — TFIIF retention in elongation complexes. A, recombinant wild type TFIIB containing a full tip region (NDKATKD as residues 57–63) or its D3 (59–61 deleted) and D7 (all 7 deleted) mutants were added to TFIIB-depleted HeLa nuclear extracts, and preinitiation complexes were isolated on an immobilized template. After transcription was begun, samples were processed at the indicated times and probed using antibody against the large subunit of IIF, Rap74. B, quantitative analysis of three independent experiments shows Rap74 retention at the indicated times. Average and S.D. values are shown for TFIIB wild type and D7 at 80 s, respectively: 85 ± 17 and 31 ± 15%.