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. 2010 Sep 29;285(51):39580–39587. doi: 10.1074/jbc.M110.171850

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FIGURE 6. — RNA levels of peroxide-inducible genes. RNAs from S. pombe cells overexpressing either wild type or D4 forms of TFIIB were reverse-transcribed, amplified by PCR, and viewed on agarose gels. A, the top panel refers to wild type, and the bottom panel refers to D4. Genes assayed were: lane 1, apt1; lane 2, atf1; lane 3, ctt1; lane 4, gpa2; lane 5, gpx1, lane 6, hsr1; lane 7, pap1; lane 8, prr1; lane 9, sty1; lane 10, trr1; lane 11, trx1. B, data are from three independent experiments. apt1 was reduced to 64 ± 2, and trx1 was reduced to 60 ± 4% in D4 compared with wild type. C, RNAs from non-inducible genes, gpd3 (lanes 1 and 3) and act1 (lanes 2 and 4), were used as controls. Top row is higher exposure of bottom row.