FIGURE 6.

Histone methyltransferases and acetyltransferases affect Myc-dependent processes. A, down-regulation of potentially dHCF-associated HATs (ATAC complex) and HMTs moderately reduces the activity of the Myc-dependent luciferase reporter. Luciferase assays were carried out in duplicate 48 h after co-transfection of the indicated dsRNAs. B, suppression of Myc-induced wing defects by co-expression of ash2- or GCN5-dsRNAs. Neither of these RNAi-transgenes had a strong effect on wing morphology when expressed on their own (although a second independent UAS-GCN5-IR insertion, expressing the same dsRNA, produced strongly deformed wings on its own upon overexpression by ap-GAL4, and it also did not rescue the Myc overexpression defect, presumably because it down-regulated GCN5 too strongly). The experiment was carried out and evaluated as indicated in Fig. 3B (n = 21, 26, 31 for lacZ, GCN5, ash2, respectively). Note that the full composition of the Drosophila dHCF-HMT complex has not been published, and some of the listed proteins were included because of their similarity to vertebrate or yeast HMT-components (Ash2/Hs Ash2L; CG40531/Sc Set1; Ash1, Trx, Trr/SET-domain containing HMTs; Mnn1/Hs Men1; wds/Hs WDR5; CG5585/Hs RBBP5; CG6444/Sc Sdc1); the ATAC components are taken from Refs. 25, 45.