FIGURE 1.

Effect of DTT on GSH and GSSG levels in isolated brain and liver mitochondria. Liver mitochondria were isolated using differential centrifugation (DC) or discontinuous Percoll gradient (DPG) as described under “Experimental Procedures.” Brain mitochondria were isolated using discontinuous Percoll gradient. Following isolation, mitochondria were incubated at 37 °C for 5 min with or without DTT (1 mm). Mitochondria were subsequently pelleted, resuspended in 5% metaphosphoric acid, and centrifuged once more. GSH (A) and GSSG (B) levels were measured in the supernatant using HPLC with electrochemical detection. Shaded bar, no DTT added; black bar, plus 1 mm DTT.