TABLE 3.

Mitochondrial glutathionylated proteins and enzyme activities: effect of substrate supplementation

Freshly isolated mitochondria with or without supplementation with substrates (2.4 mm glutamate, 2.4 mm malate, and 0.7 mm ADP) were subjected to either freeze/thaw (broken mitochondria) or sonication (submitochondrial particles), and the ATP synthase and succinyl-CoA transferase activities were measured as described under “Experimental Procedures.”

	Activity
	nmol/min/mg of protein
ATP synthase (−d[NADH]/dt)
Glutathionylated mitochondria	582 ± 48
Substrate-supplemented mitochondria	1870 ± 12
Succinyl-CoA transferase (+d[Acetyl-CoA]/dt)
Glutathionylated mitochondria	294 ± 84
Substrate-supplemented mitochondria	666 ± 18