FIGURE 10.
Mass spectra of 15-HETE formed in the reaction of A451G eLOX3 with 13S-HPODE and arachidonic acid. A, reaction with [16O]13S-HPODE in H216O. B, reaction with [18O]13S-HPODE in H216O. C, reaction with [16O]13S-HPODE in 83% H218O. The mass spectra were obtained in LC-ESI-MS analysis using a Phenomenex C18 column (2.6 μm, 0.3 × 10 cm), a flow rate of 0.3 ml/min, and a solvent system of methanol/water (80:20, by volume), containing 10 mm ammonium acetate. MS analysis detected negative ions in the range from m/z 200 to 700.