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RP-HPLC analysis of the products from anaerobic reactions with 15S-HPETE. A, A451G eLOX3. B, soybean LOX-1. C, wild-type eLOX3. RP-HPLC analysis used a Waters Symmetry C18 column (0.46 × 25 cm), a flow rate of 1 ml/min, and a solvent system of methanol/water/acetic acid (80:20:0.01, by volume).
