FIGURE 2.

Nuclear HDAC activity is decreased after liver I/R and hypoxia. A, nuclear protein was extracted from mice livers subjected to a time course of ischemia/reperfusion. HDAC activity was determined by colorimetric assay. *, p < 0.05. Assay shown is representative of three experiments with similar results. B, Western blot of acetylated (Lys-9 and Lys-18) and total H3 in sham and 1 h I/R mice. C, hepatocytes were exposed to hypoxia (1% O₂) for 1, 4, 8, and 24 h and nuclear protein was analyzed for HDAC activity. *, p < 0.05. Assay shown is representative of three experiments with similar results.