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. 2010 Oct 2;285(51):39922–39934. doi: 10.1074/jbc.M110.178087

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FIGURE 3. — Mutated DHC enhanced the interaction of DIC and DLIC1 with DHC. A, microtubule-associated proteins were prepared from newborn mouse brains. Dynein complex was enriched in the microtubule pellet (MT proteins). Equal total protein was loaded in each lane (MT proteins in first two lanes; input in last two lanes). The blot was probed with antibodies against DHC, p150^Glued/135, DIC1/2, DIC1, DIC2, DLIC1, DLIC1/2, and Tctex-1. B, quantification of dynein-complex composition in MT-associated proteins and comparison of +/+ with Loa/Loa in A. Two-way ANOVA followed by Bonferroni post-tests revealed that the fold change in the p150^Glued/DHC ratio in MT-bound dynein-dynactin complex is not statistically different between +/+ and Loa/Loa; but those of DIC^1/2/DHC, DIC1/DHC, DIC2/DHC, DLIC^1/2/DHC, and DLIC1/DHC ratios are significantly higher in Loa/Loa compared with those in +/+.