FIGURE 5.

Mutant DHC interactions with DIC-2A isoform is significantly enhanced in vivo. Firefly luciferase reporter construct was cotransfected with p53 bait alone, SV40 large T antigen (SVT) prey alone, or both constructs together in HEK293T cells as controls. Cotransfection of p53 bait and SV40 large T antigen prey resulted in marked increase in the reporter gene activity. Cotransfection of the constructs harboring wild type or mutant DHC bait with individual DIC isoform preys produced varying levels of the reporter gene activation, but mutant DHC interaction with DIC-2A isoform gave rise to a significant increase (p < 0.01) in the reporter gene activation when compared with the interaction between wild type DHC and this isoform.