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. 2010 Oct 2;285(51):39922–39934. doi: 10.1074/jbc.M110.178087

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FIGURE 6. — KLC1 is up-regulated in Loa. A, RNA was extracted using a NucleoSpin II RNA extraction kit from at least three E13 brains from each genotype. cDNA was generated with the Promega reverse transcription system, SYBR Green master mix, and a Stratagene Mx4000 qPCR system was used for analysis. qPCR analysis revealed up-regulation of KLC1 in +/Loa (p = 0.006) and Loa/Loa (p < 0.0001) compared with wild type and between +/Loa and Loa/Loa (p = 0.0003). B and C, protein analysis also revealed a trend for up-regulation in +/Loa and Loa/Loa; n = 4. E13 brains were homogenized in homogenization buffer and then centrifuged at 16,000 × g for 10 min at 4 °C. Equal quantities were loaded onto NuPAGE 4–12% gradient gel for immunoblotting.