FIGURE 9.

Galantamine increased Aβ clearance in the brains of APdE9 mice. A and B, brain sections of vehicle-treated (A) or galantamine-treated (B) APdE9 mice were immunostained with anti-Aβ antibody. Scale bar, 500 μm. C–F, in the brains of vehicle-treated (C and E) and galantamine-treated (D and F) APdE9 mice, immunoreactivities of α7 nAChRs (C and D, green) and APL-binding site (E and F, FK1; green) were co-localized (arrows) with microglia (Iba1; red) accumulated on Aβ plaque (FSB; blue). Scale bar, 20 μm. G and H, in the TBS-extracted fractions and FA-extracted fractions prepared from the brains of APdE9 mice, amounts of Aβ40 (G) and Aβ42 (H) were measured by ELISA. *, p < 0.05; **, p < 0.01 versus vehicle-treated APdE9 mice. Gal, galantamine. The data represent the means ± S.E. of seven samples in each group.