FIGURE 5.

Abl kinases are required for invadopodia formation. A, NIH3T3-SrcY527F cells were treated with DMSO or with 10 μm STI571 for 16 h, fixed and co-stained for cortactin (red, left panels) and F-actin (green, middle panels). Merged fields (right panels) demonstrate co-localization between cortactin and F-actin at invadopodia. B, NIH3T3-SrcY527F cells were transfected with either control or Abl and Arg siRNAs for 48 h, and then fixed and co-stained for cortactin and F-actin. C, lysates from cells transfected with control (Con) or Abl/Arg (A/A) siRNAs were analyzed by Western blotting for the indicated proteins.