FIGURE 7.

Syndecan-2 does not interact with TG2 directly but requires activation by PKCα and can be blocked by ROCK inhibitor. A, co-immunoprecipitation of syndecan-2 and TG2 was performed as described under “Experimental Procedures,” whereas syndecan-4 antibody was used as the positive control antibody for the pull-down procedure. gplTG was loaded as the internal standard. B, co-immunoprecipitation of PKCα and syndecan-2 using syndecan-2 antibody and detection of PKCα by Western blotting. Samples from wild type (wt), syndecan-4 Y188L mutant (Y188L), and syndecan-4 null (SDC-4 ko) MEF cells were prepared as described under “Experimental Procedures.” C, after treatment with ROCK inhibitor Y27632 (10 mm) or DMSO treatment (0.1%), MEF cells were seeded on FN or TG-FN with RAD or RGD peptide. The percentage value of non-treated attached and spread cells on FN was used as 100%.