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. 2010 Oct 7;285(51):40212–40229. doi: 10.1074/jbc.M110.123703

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Overexpression of syndecan-2 or syndecan-4 further enhances the compensatory effect of TG-FN complex. A and C, Western blotting of syndecan-2 or syndecan-4 in wild type and syndecan-2- or syndecan-4-overexpressing T98G glioblastoma cells. Following cell surface biotinylation as described under “Experimental Procedures,” α-tubulin is used to control for equal loading of proteins. B and D, cell adhesion assay for syndecan-2 (SDC-2 T98G) or syndecan-4 T98G (SDC-4 T98G) cells was undertaken in the presence of RAD or RGD peptide. Mean cell attachment and spreading are expressed as the percentage of control values of wild type T98G (wt T98G) or FN (which represents 100%) ± S.D. in the presence of RAD peptide.