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. 2010 Oct 18;285(51):40322–40332. doi: 10.1074/jbc.M110.137604

FIGURE 3.

FIGURE 3.

CS inhibits AM efferocytosis in part by de novo ceramide synthesis via SPT. A, effect of aqueous extract of ambient AC or CS (1, 3, 5, and 10% v:v; 4 h) on rat AM efferocytosis, measured by flow cytometry (means ± S.E.; n = 10; *, p < 0.005 versus control). B, time-dependent effects of CS (3% CS extract v:v) on rat AM efferocytosis, measured by flow cytometry; means ± S.E.; n = 3; *, p < 0.05 versus untreated control (UT). C, total ceramides 24 h after removal of rat AM from treatment with CS (3%; 4 h), measured by tandem mass spectrometry, followed by normalization by intracellular inorganic phosphorus content; means ± S.E.; n = 4; *, p < 0.01 versus control. D and E, rat AM efferocytosis of PI-labeled apoptotic Jurkat cells following CS exposure (3–5%; 4 h) and specific ceramide synthesis inhibitors myriocin (My; 50 nm; 2 h), fumonisin (FB1; 5 μm; 2 h), or GW4869 (20 μm; 30 min). Engulfment was assessed after 4 h of CS exposure (D) or 24 h after removal of AM from the CS treatment (E) and quantified by flow cytometry (means ± S.E.; n = 3; *, p < 0.05 versus AC).