FIGURE 5.
Current and fluorescence responses recorded from GABAARs incorporating TMRM-labeled α1-N20′C subunits. A, current and fluorescence traces recorded from α1-N20′C β2 GABAARs in response to the indicated concentrations of GABA, β-alanine, and gabazine. B and C, comparison of ΔImax (black) and ΔFmax (red) responses for GABA, β-alanine, and gabazine at the α1-N20′C β2 (B) and α1-N20′C β2 γ2 (C) GABAARs. Data are normalized to mean GABA values. Note the complete absence of gabazine responses in B and C. D, current and fluorescence traces recorded from α1-N20′C β2 GABAARs and α1-N20′C β2 γ2 GABAARs in response to the indicated concentrations of GABA and diazepam (diaz). GABA was applied at the EC10 concentration for each receptor. Error bars, ± S.E.