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. 2010 Oct 15;285(51):40397–40408. doi: 10.1074/jbc.M110.138776

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FIGURE 3. — Co-purification of Myc tagged ParE2 with His-tagged ParD2. A, His-ParD2 from JY307, E. coli BL21 co-expressing His-ParD2 and ParE2-Myc (lysate) was affinity-purified using Ni-NTA resin. The starting lysate flowthrough (FT), washes, and eluted fractions were analyzed by Western blotting with anti-His and anti-Myc antibodies. B, cell lysate from JY281, E. coli DH5α-expressing ParE2-Myc were processed as in A and then analyzed using anti-Myc antibody. C, His-tagged ParD2 (∼50 relative units (R.U.) was coupled to a Ni-NTA chip, and native ParE2 was injected at the indicated concentrations, expressed in terms of monomer.