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The effect of ParE2 on gyrase supercoiling (A) and relaxation (B) of plasmid DNA substrates. The reactions were performed as described under “Experimental Procedures” except in B the reaction was stopped with SDS and proteinase K. In A, the substrate was relaxed pBR322 DNA, and in B the substrate was negatively supercoiled pCB182. M indicates 1Kb plus DNA ladder.
