Table 1.
Molecular assays used to detect the presence of the CaMV 35S promoter and NOSt in a subset of leaf maize samples collected in 2001 from the Sierra Juárez, Oaxaca, Mexico. For the criteria to score presence (1) or absence (0) of the expected PCR band in the gels see text
| IE-UNAM |
Genetic ID |
|||||
|---|---|---|---|---|---|---|
| PCR | SB | PCR | Qn-PCR | |||
| Locality | sample code | 35S (N) | 35S | 35S | NOSt | 35S |
| Positive samples | ||||||
| 7 | 1 | 1 | 1 | 1 | 1 | 0.60% |
| 7 | 2 | 1 | 1 | 1 | 1 | N.A. |
| 7 | 3 | 1 | 0 | 1 | 1 | N.A. |
| 7 | 4 | 1 | 1 | 1 | 1 | N.A. |
| 7 | 5 | 1 | — | 1 | 1 | 100% |
| 11 | 6 | 1 | 1 | 1 | 1 | N.A. |
| 23 | 7 | 1 | 1 | 1 | 1 | N.A. |
| 23 | 8 | 1 | 1 | 1 | 1 | N.A. |
| 23 | 9 | 1 | 1 | 1 | 1 | N.A. |
| 23 | 10 | 1 | 1 | 1 | 1 | N.A. |
| Negative controls | ||||||
| N.A. | 11 | 0 | 0 | 0 | 0 | N.A. |
| N.A. | 12 | 0 | 0 | 0 | 0 | N.A. |
| N.A. | 13 | 0 | 0 | 0 | 0 | N.A. |
| Positive Controls | ||||||
| N.A. | MON810 | 1 | 1 | 1 | N.A. | 120% |
| N.A. | NK603 | 1 | 1 | 0 | 1 | N.A. |
PCR, Qualitative PCR; SB, Southern Blot; Qn-PCR, Quantitative PCR; N, Native Taq Polymerase; N.A., Not Applicable. We present only three of the 10 negative samples assayed.