Figure 5.

Comparison of the empirical Hill coefficient n for A-riboswitch folding with a direct measurement of ΔΓ₂₊. (circles), plot of Hill coefficients n vs. titration midpoints (C₂₊) taken from Table 1. Subscripts of n refer to the wavelength at which titration data were collected. (dashed line), ΔΓ₂₊ calculated from measurements of Γ₂₊ for folded and partially unfolded RNA (Figure 6). Error bars are shown at selected C₂₊ for comparison with n values.