Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 6;38(22):8277–8283. doi: 10.1093/nar/gkq650

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Optimizing the ratio and lengths of frameshift-inducing RNA oligonucleotides. (a) An amount of 0.05 pmol of SF468 mRNA were mixed with 0.05, 0.25, 1.25, 6.25 and 31.25 pmol of RNA6 and RNA9 oligonucleotides, respectively. Mixtures were subsequently translated in the presence of ³⁵S-methionine and the labeled proteins were examined by 13% SDS–PAGE. Frameshift efficiencies [FS (%)] were calculated after quantification and correction of in-frame and frameshifted product. (b) A total of 625 M excess of different lengths of RNA oligos (RNA6, 9, 12, 15, 18, respectively) and control without added oligonucleotide were mixed with 0.05 pmol of SF468 mRNA. Mixtures were translated and examined by 13% SDS–PAGE. In-frame and frameshifted protein products are indicated by NFS and FS, respectively. Frameshifting efficiency [FS (%)] and standard deviation (SD) of three independent duplicate assays are indicated below each lane.