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. 2010 Aug 12;38(22):8328–8337. doi: 10.1093/nar/gkq681

Figure 1.

Figure 1.

TNd/c BART mutants and reporter plasmid psiCHECK2-BART2. (A) Genome organization of wild-type TBEV virus strain Neudoerfl (TNd/c). 5′-NCR, 5′-non-coding region; 3′-NCR, 3′-non-coding region. The positions of the ‘variable’ and conserved ‘core’ regions of the 3′-NCR are indicated. (B) EBV miR-BART2 precursor in the predicted secondary structure (miRBase accession number: MI0001068) flanked by 30 nt of EBV B95-8 sequence on either side. The sequence of the precursor of miR-BART2 is highlighted in red and blue, with the red region corresponding to the mature miR-BART2. (C) Top: Schematic diagram of the 3′-NCR of parental virus TNd/c and the derived TNd/c BART mutants. Below: IF staining of infected cells with rabbit anti-TBEV serum 24 h postinfection. (D) Mature miR-BART2 (red) and the corresponding target sequence in the 3′-UTR of the EBV BALF5 mRNA (green) that was inserted into the 3′-UTR of the Rluc mRNA gene of the parental vector psiCHECK-2. The fluc expressed from the HSV-TK promoter served as an internal standard. Promoters are indicated by flags: SV40, simian virus 40; HSV-TK, herpes simplex virus thymidine kinase. Diagrams are not drawn to scale.