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eap1Δ and caf20Δ cells have nitrogen utilization defects. Cells complemented for auxotrophic markers were grown in SC complete medium to A600 = 0.6 washed and diluted to A600 = 0.1, then 10-fold serially diluted and spotted (3 µl) onto the indicated media. Growth was scored on a scale of 0 (none), +/− (minimal) to 3+ (wild-type).
