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Cm resistance of RFzero-q with the wild-type cat gene and the cat(3Am) and cat(10Am) mutant genes. (A) The amino acid sequence of the cat(10Am) gene is shown with X’s representing the positions of UAG. The asterisks show the UAG positions in cat(3Am). (B) Each third of the upper and lower halves of each plate shows the growth of the HST08 and RFzero-q strains, respectively, transformed with the indicated cat genes. Cm concentrations are indicated above the plates.
