Table 1.
Plasmids used in this study
| Plasmids | Description or relevant characteristics | Reference |
|---|---|---|
| pKK232-8 | Cloning vector with a promoterless cat used for promoter selection. | (53) |
| pKK-IntI1 | 349-bp SalI-HindIII PCR fragment amplified from S. marcescens SCH909 genomic DNA (primer pair IntI1-SalI and aadB-HindIII) containing part of intI1 and the nucleotide sequence up to the initiation codon of the aadB::S.ma.I2 gene cassette and cloned into pKK232-8 digested by SalI-HindIII. | This study |
| pKK-SmaI2 | 2095-bp SalI-HindIII PCR fragment amplified from S. marcescens SCH909 genomic DNA [primer pair SmaI2-SalI and ant(3″)Ii-HindIII] containing the entire group II intron S.ma.I2 (1971 bp) and the nucleotide sequence up to the initiation codon of the ant(3″)-Ii-aac(6′)-IId gene cassette and cloned into pKK232-8 digested by SalI-HindIII. | This study |
| pKK-In | 2900-bp SalI-HindIII PCR fragment amplified from S. marcescens SCH909 genomic DNA [primer pair IntI1-SalI and ant(3″)Ii-HindIII] containing part of intI1, the aadB::S.ma.I2 gene cassette and the nucleotide sequence up to the initiation codon of the ant(3″)Ii-aac(6′)-IId gene cassette and cloned into pKK232-8 digested by SalI-HindIII. | This study |
| pKK-InΔSmaI2 | Clone derived from pKK-In by PCR (primer pair Sm909–3947.for and Sm909–1507.rev) to remove the group II intron S.ma.I2 (‘Materials and Methods’ section). | This study |
| pKK-NeI1-P1out | 200-bp PCR fragment amplified from N. europaea genomic DNA (primer pair NeI1-prom.for and NeI1-prom.rev) containing part of the group II intron N.e.I1 (base positions 1–200 in N.e.I1) and cloned into pKK232-8 digested by SmaI. | This study |
| pKK-GsI1-P1out | 200-bp PCR fragment amplified from G. sulfurreducens genomic DNA (primer pair GsI1-prom.for and GsI1-prom.rev) containing part of the group II intron G.s.I1 (base positions 1–200 in G.s.I1) and cloned into pKK232-8 digested by SmaI. | This study |
| pKK-ShbaI2-P1out | 200-bp PCR fragment amplified from S. baltica genomic DNA (primer pair ShbaI2-prom.for and ShbaI2-prom.rev) containing part of the group II intron Sh.ba.I2 (base positions 1–200 in Sh.ba.I2) and cloned into pKK232-8 digested by SmaI. | This study |
| pKK-SmaI2-P2out | 383-bp SspI-BglII restriction fragment digested from the pUCSmI plasmid (36) containing part of the group IIC intron S.ma.I2 (base positions 288–675 in S.ma.I2) and cloned into pKK232-8 digested by SmaI by the TA-cloning method. | This study |
| pLQ872 | Weak Pc promoter from integron In0 (pVS1) cloned in pKK232-8. | (1) |
| pLQ876 | Strong Pc promoter from integron In4 (Tn1696) cloned in pKK232-8. | (1) |
| pLQ880 | 96-bp HindIII-BamHI fragment of tac promoter cloned in pKK232-8. | (1) |