Table 2.

Expression of cat reporter gene in E. coli from promoter sequences within various cloned DNA fragments from S. marcescens SCH909 integron #2

Clonea	DNA fragments from integron #2 cloned in pKK232-8	Promoter	Cm acetylated (%)b	Ratio relative to pKK-In
Nonec	NA	NA	0.3 ± 0.1	0.04
pKK232-8	none	None	0.4 ± 0.1	0.05
pKK-IntI1	Partial 5′-CS	Pcweak-P2 in 5′-CS	28.1 ± 5.2	3.75
pKK-InΔSmaI2	Partial 5′-CS + aadB-attC	Pcweak-P2 in 5′-CS	27.3 ± 1.6	3.64
pKK-In	Partial 5′-CS + aadB::S.ma.I2-attC	Pcweak-P2 in 5′-CS + putative Pout in S.ma.I2	7.5 ± 0.5	1.00
pKK-SmaI2	S.ma.I2-attC	Putative Pout in S.ma.I2	6.7 ± 1.7	0.89

^aFor detailed information about these clones see Table 1.

^bMeans ± SD of three independent experiments.

^cTris–HCl (1 mM [pH 7.6]) was added to the reaction mix instead of crude cell extracts.

NA, not applicable