Skip to main content
. 2010 Aug 16;38(22):8061–8071. doi: 10.1093/nar/gkq717

Figure 4.

Figure 4.

AUF1 controls FGF9 mRNA stability. (A) Western blots showing AUF1-specific siRNA, but not the scramble control (SC) or AUF1-specific siRNA control (AC), knocks down AUF1 protein. Mock is HEK293 cells treated with transfecting reagent only and α-tubulin was used as a control. (B) Luciferase reporter activities in empty vector (pGL3-P), full length (FGF9 3′-UTR), and mutant (mutARE) after AUF1 or scramble siRNA knockdown were shown. (C) Endogenous FGF9 mRNA levels in HEK293 cells following AUF1 or scramble siRNA knockdown. (D) Real-time RT-qPCR measurements showing the half-life of endogenous FGF9 mRNA with or without siRNA knockdown. Results are normalized to time zero for each construct. All data are shown as mean ± SEM of 4–6 independent experiments. **P < 0.01; ***P < 0.001.