Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 16;38(22):8061–8071. doi: 10.1093/nar/gkq717

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — AUF1 p42 isoform selectively downregulates FGF9 mRNA expression. (A) Pull-down assay showing that p42^AUF1 is the major isoform pulled down by biotin-labeled FGF9 ARE probe. Arrowhead indicates the position of each AUF1 isoform. Note that the mutant is markedly weaker than the wild type. Input and probe only indicate positive and negative controls. (B) Western blots using anti-Myc antibody showing the expression of indicated recombinant AUF1 proteins. HEK293 and pcDNA3.1 refer to cells without transfection and cells transfected with empty vector, respectively. (C) Endogenous FGF9 mRNA levels relative to control (pcDNA3.1) in cells overexpressing the AUF1 isoforms p37, p40, p42 and p45. **P < 0.01.