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. 2010 Aug 16;38(22):8061–8071. doi: 10.1093/nar/gkq717

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — AUF1 knockdown sustains the PGE₂-medicated induction of FGF9 mRNA in human primary endometrial stromal cells. (A) Western blots showing successful AUF1 knockdown by AUF1-specific shRNA (B01, B03 and C03), but not the Luc control shRNA. The Null indicates cells without shRNA treatment. (B) Western blots showing AUF1 protein expression in whole-cell lysates isolated from stromal cells after PGE₂ treatment. (C) FGF9 mRNA levels measured at indicated times after PGE₂ treatment in stromal cells. Ethanol (EtOH) was used as control treatment. (D) Experiment similar to that shown in C, but with AUF1 knockdown by AUF1_B03 shRNA. All data are shown as mean ± SEM of 4–6 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (E) Western blots showing the distribution pattern of AUF1 protein in stromal cells after PGE₂ treatment. α-Tubulin and LaminA/C used as loading controls for proteins isolated from cytoplasmic and nuclear fractions, respectively.