Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Aug 19;38(22):8219–8230. doi: 10.1093/nar/gkq737

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Effects of Rv1495 on the single-stranded DNA cleavage activity of MtbTopA. 5′-end-labeled 32-mer oligonucleotide (5′-CAGTGAGCGAGCTTCCGCTTGACATCCC AATA-3′) (27) was used as a specific single-stranded DNA substrate. DNA-binding and cleavage assays of MtbTopA were performed as described under ‘Materials and Methods’ section. (A) DNA-binding activity assays for MtbTopA, Rv1495 and Rv0054 (a positive control). The protein concentrations were indicated on top of the panels. (B) ssDNA cleavage assays for MtbtopA (left panel) and the effects of Rv1495 (right panel). The 32-mer substrates and 19-mer cleavage products were indicated by arrows. (C) The effects of the denatured Rv1495 protein (left panel) or GST (right panel) on the cleavage activity of MtbTopA.