Table 7.

Experimental values for G-quartet formation in Arabidopsis RNA

GQS Motif	Gene IDa	K+1/2 (mM)b	Tm (°C)c
			Li+	Na+	K+
G3 L221	At1g07180	8.0 ± 0.7	54	62	>85
G3 L444	At5g53580	42 ± 5	30	45	74
G3 L444 + FLANKd	At5g53580	220 ± 70	NDe	ND	ND
G2 L111	At2g39320	30 ± 2	32	43	>85
G2 L444d	At1g44020	316 ± 1	ND	ND	62

Provided are thermodynamic parameters for G-quartet formation for RNA oligonucleotides from representative Arabidopsis genes. See ‘Materials and Methods’ section for full sequences.

^aGene ID identifies the particular gene from the Arabidopsis genome and sequences are provided in ‘Materials and Methods’ section.

^bK⁺_1/2 (K⁺ concentration needed to fold half the RNA) values were determined by CD titrations and using Equation (1).

^cT_m (melting temperature) values were determined by UV thermal melts at 100 mM monovalent salt concentration using the chloride salt.

^dMelts for these oligonucleotides were performed at 1M salt concentration owing to their higher K⁺_1/2 values.

^eND indicates that the T_m value could not be determined due to absence of a well defined folding transition.