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. 2010 Oct 4;38(22):e202. doi: 10.1093/nar/gkq836

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Femtosecond laser irradiation induces a phase dark spot that results in the co-localization of Nbs1 and in the formation of γH2AX on the surrounding damaged area. Shown above (a–d) and corresponding zoomed in figures (e–h). (a and e) Pre-irradiation images. (b and f) Post-irradiation image shows a phase paling at site exposed to laser. (c and g) After 40 s there is the appearance of a phase dark material (arrow). (d and h) Post fixation images. Cells were fixed and stained for phosphorylated γH2AX (k, green) and for Nbs1 (j, red) 8 min after laser irradiation. γH2AX is observed to surround the damage site (k) and (l; an overlap between h and k). Nbs1 colocalizes with the phase dark material as shown on (i; an overlap between h and j). (m) An overlay between Nbs1(j), yH2AX(k) and phase image (h) shows the position of these proteins with respect to the dark material. (n) A merged image between (j) and (k) shows that Nbs1 and yH2AX do not completely overlap.