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. 2010 Oct 4;38(22):e202. doi: 10.1093/nar/gkq836

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Cells fixed prior to the formation of the phase dark spot do not show strong Nbs1 localization at the damage site. (a) Pre laser. (b) Two seconds post-laser paling is observed on two chromosomes damaged by the laser (arrows). This cell was fixed 3 s post-laser. (c) DAPI staining shows lack of fluorescence in areas damaged by the laser (arrows). (d) Nbs1 demonstrates background fluorescence in chromosomal lesions (arrows and insets). (e) Different cell 1 min 35 s post-laser shows phase dark spot in damaged region (arrow). (f) Post-fixation image of cell 21 min and 25 s post-laser. (g) DAPI staining shows lack of fluorescence in damaged area. (h) Nbs1 immunofluorescence staining shows a bright fluorescing spot at the lesion.